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Applied Biosystems™ TaqMan™ SNP Genotyping Assay, human
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Description
Includes
Two unlabeled PCR primers: (forward and reverse primers at 900nM final concentration); One Applied Biosystems VIC™ dye: MGB labeled probe detects the Allele 1 sequence (probes at 200nM final concentration); One Applied Biosystems 6FAM™ dye: MGB labeled probe detects the Allele 2 sequence (probes at 200nM final concentration)
- Large selection of SNP assays advances validation and screening studies for disease association and population stratification applications
- Easy-to-use, single-tube format
- Robust assay design provides highly accurate, reproducible and reliable results
- Simple workflow enables quick results
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'‐nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.
Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
- Tested—all human SNP genotyping assays are functionally tested to ensure allelic discrimination
Approximate ship time
4–6 days in North America and 6–10 days in Europe
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).
All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)
*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.
Specifications
Specifications
| 3'Primer Modification | None |
| 5'Primer Modification | None |
| Concentration | 80X |
| Content And Storage | 1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers). Store at -15 to -25°C. |
| Description | VIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2 |
| Form | Frozen |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals. |
| Green Features | Sustainable packaging |
| Internal Probe Modification | VIC™ (5'), FAM (5'), MGB (Minor Groove Binder) (3') |
| Label or Dye | FAM, VIC |
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For Research Use Only. Not for use in diagnostic procedures.
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