Invitrogen™ Human SIK3, GST Tag Recombinant Protein

SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. Recombinant human SIK3 (1-307) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Brand:  Invitrogen™ PV6404

Product Code. 15419033

Description

The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm. Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer. There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer. SIK3 is a novel tumor-associated antigen (TAA).

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Specifications

• For Use With (Application): Kinase Assay
• Quantity: 100μg
• Protein Length: aa 59-365
• Recombinant: Recombinant
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Expression System: Baculovirus
• Protein Form: Recombinant, Fragment
• Protein Subtype: Serine/Threonine Kinases

• Molecular Weight (g/mol): 61.7 kDa
• Gene Alias: QSK, SIK-3, L19, FLJ12240, KIAA0999
• Species: Human
• Protein Tag: GST Tag
• Kinase Group: Serine/Threonine Kinase
• Protein Family: Kinases & Inhibitors
• Form: Liquid