Invitrogen™ Human SIGLEC9 ELISA Kit

Product Code. 17175559

Brand: Invitrogen™ EHSIGLEC9X5

Description

ELISA

The Human Siglec-9 ELISA quantitates Hu Siglec-9 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Siglec-9. Principle of the method The Human Siglec-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

SIGLEC9 (Sialic acid-binding Ig-like lectin 9) is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It contains two Ig-like C2 type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. It preferentially binds to alpha2, 3-or alpha2, 6-linked sialic acid. The sialic acid recognition site may be mased by cis interactions with sialic acids on the same cell surface.

Specifications

• Accession Number: Q9Y336
• Assay Range: 0.123 to 30 ng/mL
• Assay Sensitivity: 130 pg/mL
• Calibration Range: 130 pg/mL to 30 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 27180
• Gene Symbol: CD329,CDw329,FOAP-9,OBBP-LIKE,siglec-9
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: UNQ668/PRO1302, CD329, CDw329, FOAP-9, OBBP-LIKE, siglec-9
• Target: SIGLEC9
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins