Invitrogen™ SIGLEC H Monoclonal Antibody (eBio440c), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15236907	Invitrogen™ 14-0333-81	50 μg
15246907	Invitrogen™ 14-0333-82	100 μg

Description

Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6. Applications Tested: This eBio440c antibody has been tested by blocking staining of the direct conjugate of eBio440c. This can be used at less than or equal to 2 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that direct conjugates such as cat. 11-0333 be used rather than purified eBio440c antibody when performing flow cytometric analysis. Purity: Greater than 90%, as determined by SDS-PAGE.

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Specifications

• Antigen: SIGLEC H
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
• Gene Symbols: Siglech
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Siglech

• Clone: eBio440c
• Applications: Flow Cytometry, Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: 0
• Gene ID (Entrez): 233274
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.