Invitrogen™ SIAH2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5105291

Product Code. 16661735

Description

Antibody detects endogenous levels of total SIAH2.

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

Specifications

• Antigen: SIAH2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SIAH2
• Gene Alias: AA415433; E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; hSiah2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 2; seven in absentia homolog 2; siah E3 ubiquitin protein ligase 2; SIAH2; siah-2; Sinh2
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140593, 20439, 6478
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O43255, Q06986, Q8R4T2
• Gene Symbols: SIAH2
• Immunogen: A synthesized peptide derived from human SIAH2(Accession O43255), corresponding to amino acid residues F246-C296.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG