Invitrogen™ SF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA579984

Product Code. 15985545

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human A549 whole cell, human THP-1 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: Human Lung Cancer tissue, rat small intestine tissue IHC-F: Rat Spleen tissue. ICC/IF: U87 cell, Hela cell. Flow: 293T cell.

Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: SF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SF1
• Gene Alias: BBP; D11S636; M ZFM1; Mammalian branch point-binding protein; MBBP; MZFM; SF; SF1; Splicing factor 1; transcription factor ZFM1; WBP4; wu:fc09f06; ZCCHC25; ZFM1; Zfp162; Zinc finger gene in MEN1 locus; zinc finger protein 162; ZNF162
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117855, 22668, 7536
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: Q15637, Q64213
• Gene Symbols: SF1
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1 (11-30aa DFPSKKRKRSRWNQDTMEQK).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG