SerpinB9 Antibody (7D8) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18095254	Novus Biologicals NB100-64359-0.025MG	0.025 mg
18085784	Novus Biologicals NB100-64359	0.1 mg

Description

SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: SerpinB9
• Concentration: 1.0 mg/mL
• Applications: Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Immunology
• Gene Accession No.: P50453
• Gene ID (Entrez): 5272
• Immunogen: Recombinant PI-9 produced in P. pastoris.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 7D8
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse (Negative), Porcine (Negative), Human
• Gene Alias: CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
• Gene Symbols: SERPINB9
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).

For Research Use Only