Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Brand:  Cytiva 17-0719-01

Product Code. 10714592

Description

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Composed of crosslinked 6% agarose beads
• Reproducible
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

SP Sepharose:

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.18 to 0.25mmol H⁺/mL medium
• Binding capacity: 70mg ribonuclease A/mL medium

CM Sepharose:

• Ligand: Carboxymethyl
• Ion Exchanger Type: Weak cation exchanger
• Ionic Capacity: 0.09 to 0.13mmol Hl⁺/mL medium
• Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications

• Quantity: 500 mL
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
• Anion Exchange Type: Weak CatIon Exchanger
• Binding Capacity: 50 mg Ribonuclease A/mL mediμm
• Flow Rate: 300 to 600 cm/Hoμr
• Max. Pressure: 100 kPa
• pH Range: 4 to 13
• Ligand Type: Carboxymethyl
• Stationary Phase: Ion Exchange Phase

• For Use With (Application): For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
• Storage Requirements: 4°C to 30°C
• Anion Exchange Capacity: 0.09 to 0.13 mM Hl+/mL
• Product Type: CM Sepharose Fast Flow Media
• Particle Size: 45 to 165 μm
• Matrix: 6% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol
• Packaging Quantity: 500 mL
• Format: Bulk Media

Safety and Handling

Product Identifier

• CM Sepharose Fast Flow, 500 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.