Cytiva Sepharose™ Fast Flow Cation Exchange Media

Código de producto.	Binding Capacity	Product Type	Packaging Quantity	unitSize
10576350	70 mg Ribonuclease A/mL Medium	SP Sepharose Fast Flow Media	25 mL	25mL
10724592	70 mg Ribonuclease A/mL Medium	SP Sepharose Fast Flow Media	300 mL	300mL
10714592	50 mg Ribonuclease A/mL Medium	CM Sepharose Fast Flow Media	500 mL	500mL
10059859	50 mg Ribonuclease A/mL Medium	CM Sepharose Fast Flow Media	25 mL	25mL

Código de producto. 10576350

Marca: Cytiva 17072910

Descripción

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Composed of crosslinked 6% agarose beads
• Reproducible
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

SP Sepharose:

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.18 to 0.25mmol H⁺/mL medium
• Binding capacity: 70mg ribonuclease A/mL medium

CM Sepharose:

• Ligand: Carboxymethyl
• Ion Exchanger Type: Weak cation exchanger
• Ionic Capacity: 0.09 to 0.13mmol Hl⁺/mL medium
• Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Especificaciones

• Quantity: 25 mL
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
• For Use With (Application): For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
• Storage Requirements: 4°C to 30°C
• Anion Exchange Type: Strong CatIon Exchanger
• Anion Exchange Capacity: 0.18 to 0.25 mM Hl+/mL
• Binding Capacity: 70 mg Ribonuclease A/mL Medium
• Product Type: SP Sepharose Fast Flow Media
• Flow Rate: 400 to 700 cm/Hoμr
• Particle Size: 45 to 165 μm
• Max. Pressure: 100 kPa
• Matrix: 6% Cross-Linked Agarose
• pH Range: 4 to 13
• Storage Buffer: 20% Ethanol, 0.2 M Sodium Acetate
• Ligand Type: Sulphopropyl
• Packaging Quantity: 25 mL

Seguridad y manipulación

Nombre del producto

• SP Sepharose Fast Flow, 25 ml

Palabra de advertencia

• Warning

Clasificación

• Flammable liquid Category 3

Indicaciones de peligro

• H226-Flammable liquid and vapour.

Consejos de prudencia

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Otros peligros

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-18 per cent of the mixture consists of component(s) of unknown acute toxicity.