Cytiva Sephacryl™ S-400 High Resolution Media

Código de producto.	Quantity	unitSize
11520794	150mL	150mL

Código de producto. 11520794 Proveedor Cytiva N.º de proveedor 17060910

Descripción

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

• For separating polysaccharides, macromolecules with extended structures, and small plasmids
• Excellent purification over a wide molecular weight range
• High reproducibility due to long-term chemical and physical stability
• High flow rates and recoveries
• Well-suited to industrial-scale use
• Storage conditions: 20% ethanol; 4° to 30°C
• Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

• 1M acetic acid, 8M urea
• 6M guanidine-HCl, 1% SDS
• 2M NaCl, 24% ethanol
• 30% propanol, 30% acetonitrile
• 0.5M NaOH (for cleaning)

pH Stability

• 3 to 11 (long-term and working)
• 2 to 13 (short-term)

Physical Stability:

• Negligible volume variation due to changes in pH or ionic strength
• Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
• Antimicrobial agent: 20% ethanol

Especificaciones

• Quantity: 150mL
• Flow Rate: >150 cm/hr.
• Particle Size: 25 to 75 μm
• Bed Volume: 19 to 230L
• Product Type: Sephacryl HR Media
• Format: Solid Spherical Bead
• Diameter (Metric) Inner: 40 to 140cm
• For Use With (Application): Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laboratory and industrial scale
• Height (English): 5.9 in.
• Height (Metric): 15cm
• Max. Pressure: 100kPa
• pH Range: 3 to 11
• Support Type: Allyl Dextran and N, N'-methylenebisacrylamide
• Sample Type: Polysaccharides, macromolecules and small plasmids
• Content And Storage: 4° to 30°C

Seguridad y manipulación

Nombre del producto

• Sephacryl S-400 High Resolution, 150 ml

Palabra de advertencia

• Warning

Clasificación

• Flammable liquid Category 3

Indicaciones de peligro

• H226-Flammable liquid and vapour.

Consejos de prudencia

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Otros peligros

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.