Invitrogen™ Human SDF-1 alpha/CXCL12A ELISA Kit

Product Code. 15424963

Brand: Invitrogen™ EHCXCL12A

Description

ELISA

The Human SDF-1alpha/CXCL12A (SDF-1?) ELISA quantitates Hu SDF-1? in human serum,plasma,or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SDF-1?. Principle of the method The Human SDF-1? solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P48061
• Assay Range: 93.75 to 6000 pg/mL
• Assay Sensitivity: 80 pg/mL
• Calibration Range: 80 pg/mL to 6000 pg/mL
• Description: SDF-1a (CXCL12A) ELISA Kit, 96 Assays, 1 Kit, Human Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of SDF-1a (CXCL12A) in Samples Using a Microplate Reader
• Detectable Analytes: SDF-1 A (CXCL12A), Human SDF-1 A
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Detection Method: Colorimetric
• Product Type: ELISA
• Protein Family: Chemokine & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6387
• Gene Symbol: IRH,PBSF,SCYB12,SDF1,TLSF,TPAR1
• Host Species: Human
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Signal Transduction
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
• Target: CXCL12 alpha
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins