Purification of Monoclonal and Polyclonal Antibodies from Serum, ascites and cell culture supernatants
MiniprepA spin column Kit
Vivapure miniprep and spin columns are pre-packed centrifugal devices for purification of monoclonal and polyclonal antibodies from serum, ascites and cell culture supernatants. The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel. Vivapure miniprep & spin columns can conveniently be used in a centrifuge with swing-out rotor for standard 2.2 ml tubes. Binding capacity is about 1 mg IgG per unit. The low hold-up volume ensures high solute recovery with minimal loss by non-specific adsorption. Vivapure miniprep and spin columns can be re-used three times and are available as complete kits including buffers or as bulk packs.They are an attractive alternative to lengthy chromatographic methods such as FPLC. For larger volumes, the Vivapure Maxiprep kit is also availablity.