Invitrogen™ SARS-CoV-2 Spike Protein S1/S2 (B.1.351) His-tag Recombinant Protein

Product Code.	Quantity	unitSize
17966361	100 μg	100µg

Product Code. 17966361 Supplier Invitrogen™ Supplier No. RP88532

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. The mutations were identified in the SARS-CoV-2 varient known as 20C/501Y.V2 or B.1.351.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.

Specifications

• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: 20mM tris with 10% glycerol, 0.01% mannitol, 300mM NaCl, 5-8% trehalose, 0.01% Tween 80 and no preservative; pH 8
• Molecular Weight (g/mol): 134 kDa
• Name: SARS-CoV-2 Spike Protein S1/S2 (B.1.351) His-tag
• pH Range: 8
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein S1/S2
• Biological Activity: Immobilized human ACE2 protein (mFc tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (D80A, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) ,the EC50 of SARS-CoV-2 Spike S1+S2 (D80A, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) is 100-600 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1), amino acids Met1-Pro1213 with D80A, (LAL242-244) deletion, R246I, K417N, E484K, N501Y, D614G, A701V substitutions and a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (D80A, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) ,the EC50 of SARS-CoV-2 Spike S1+S2 (D80A, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag) is 100-600 ng/mL.
• Expression System: Baculovirus
• Form: Lyophilized
• Purity or Quality Grade: >85% as determined by SDS-PAGE.