S100B Mouse anti-Bovine, Human, Mouse, Rat, Clone: S100B/1012, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18246713	Novus Biologicals NBP2-59618-20UG	20 μg
18055496	Novus Biologicals NBP2-59618-100UG	100 μg

Description

S100B Monoclonal specifically detects S100B in Human, Mouse, Rat, Bovine samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: S100B
• Concentration: 0.2 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), SDS-Page
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cytoskeleton Markers, Neuroscience, Stem Cells
• Formulation: 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 6285
• Immunogen: Recombinant full-length human S100B protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 13 kDa

• Clone: S100B/1012
• Dilution: Western Blot 0.5-1 ug/ml, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, Immunohistochemistry-Paraffin 0.25-0.5 ug/ml, SDS-Page, Protein Array 1:100-1:2000
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine
• Gene Alias: beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta
• Gene Symbols: S100B
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta -chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lip ocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell -derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S -100 protein

For Research Use Only