Invitrogen™ RUNX2 Recombinant Rabbit Monoclonal Antibody (6H4L27), Alexa Fluor™ Plus 488

Rabbit Recombinant Monoclonal Antibody

Product Code	Brand	Quantity
30274848	Invitrogen™ 702489RP488	50 μL
30275112	Invitrogen™ 702489RP48820UG	20 μL

Description

Alexa Fluor™ Plus recombinant rabbit antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This 6H4L27 antibody has been tested by western blot and flow cytometric analysis of U-2 OS cells. This may be used for western blot at 5 μg/mL and for flow cytometry at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 494 ...

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

Specifications

• Antigen: RUNX2
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Western Blot
• Conjugate: Alexa Fluor Plus 488
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: Proprietary buffer with 0.008 Bromonitrodioxane, 0.008 Methylisothiazolone; pH 6.8
• Gene Accession No.: Q13950
• Gene ID (Entrez): 860
• Immunogen: Protein corresponding to human RUNX2 [aa235-aa400].
• Product Type: Antibody

• Clone: 6H4L27
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: RUNX2
• Gene Alias: Acute myeloid leukemia 3 protein; AKV core binding factor; Aml3; AML-3; Cbf; Cbfa1; Cbfa-1; CBF-alpha-1; CCD; CCD1; CLCD; core binding factor alpha 1; core binding factor alpha 1 subunit; core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; LOW QUALITY PROTEIN: runt-related transcription factor 2; LS3; Oncogene AML-3; Osf2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2 alpha A; PEBP2A; Pebp2a1; PEBP2aA; PEBP2-alpha A; Pebpa2a; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX 2; RUNX family transcription factor 2; Runx2; RUNX-2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit; transcription factor RUNX2; transcription factor RUNX2/CBFA1
• Gene Symbols: RUNX2
• Primary or Secondary: Primary
• Purification Method: Protein A