Invitrogen™ Rubicon Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA562974

Product Code. 15700762

Description

Immunogen sequence: RSHSDTSIAS RGAPESCNDK AKLRGPLPYS GQSSEVSTPS SLYMEYEGGR YLCSGEGMFR RPSEGQSLIS YLSEQDFGSC ADLEKENAHF S Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 64%.

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.

Specifications

• Antigen: Rubicon
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RUBCN
• Gene Alias: 1700021K19Rik; 5330403K09; baron; Beclin-1 associated RUN domain containing protein; KIAA0226; mKIAA0226; RGD1305422; RIKEN cDNA 1700021K19 gene; RUBCN; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cysteine-rich containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; SCAR15; similar to mKIAA0226 protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9711
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q92622
• Gene Symbols: RUBCN
• Immunogen: Recombinant protein corresponding to Human Rubicon. Recombinant protein control fragment (Product #RP-100145).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG