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Invitrogen™ ROCK1 Polyclonal Antibody

Product Code. 12859272
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Quantity:
100 μg
Unit Size:
100µg
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Product Code. Quantity unitSize
12859272 100 μg 100µg
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Product Code. 12859272 Supplier Invitrogen™ Supplier No. PA521130

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Description

Rabbit Polyclonal Antibody

A suggested positive control is 293 cell lysate. PA5-21130 can be used with blocking peptide PEP-1244.

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
TRUSTED_SUSTAINABILITY

Specifications

Antigen ROCK1
Applications Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% sodium azide
Gene Rock1
Gene Accession No. P70335, Q13464, Q63644
Gene Alias 1110055K06Rik; Ac2-154; Liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160ROCK; p160-ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho associated coiled-coil containing protein kinase 1; Rho kinase; Rho-associated coiled-coil containing protein kinase 1; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK1; ROCK-I
Gene Symbols Rock1
Host Species Rabbit
Immunogen A 15 amino acid peptide near the amino terminus of human ROCK1.
Purification Method Antigen Affinity Chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 19877, 6093, 81762
Target Species Human, Mouse, Rat
Content And Storage Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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