Invitrogen™ RNF2 Recombinant Rabbit Monoclonal Antibody (13H1L14)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 703252

Product Code. 16311774

Description

This antibody is predicted to react with Monkey, Mouse, Rat, Pig. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.

Specifications

• Antigen: RNF2
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q99496
• Gene Symbols: RNF2
• Immunogen: Peptides corresponding to human RNF2 [1) aa183-aa201 2) aa155-aa174].
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Clone: 13H1L14
• Conjugate: Unconjugated
• Gene: RNF2
• Gene Alias: AI326319; AI450156; AU019207; BAP 1; BAP1; BAP-1; DinG; DinG protein; E3 ubiquitin-protein ligase RING2; fa19h05; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; HIPI3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; protein DinG; RING 1B; RING 2; RING finger protein 1B; ring finger protein 2; RING finger protein BAP-1; RING1B; RING2; RING-type E3 ubiquitin transferase RING2; RNF 2; Rnf2; wu:fa19h05; wz4556; wz4566
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 6045
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid