Invitrogen™ RNAqueous™ Total RNA Isolation Kit

Artikelnummer.	Quantity	Product Type	unitSize
10075994	50 Preps	RNA Isolation Kit	Each
10176864	50 Catridges	Spin Columns	Each

Artikelnummer. 10075994 Leverandør Invitrogen™ Leverandørnr. AM1912

Beskrivelse

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10²–10⁷ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works

The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Tekniske data

• Content And Storage: • 6 mL Formaldehyde Load Dye; store at -20°C
  • 60 mL Lysis/Binding Solution; 4°C
  • 60 mL Water; 4°C
  • 4 mL Lithium Chloride Precipitation Solution; 4°C
  • 40 mL Wash Solution #1; 4°C
  • 80 mL Wash Solution #2/3 (concentrated); 4°C
  • 50 Filter Cartridges; room temperature
  • 100 Collection Tubes; room temperature
  • 10 mL Elution Solution; room temperature
• Isolation Technology: Spin Column (Glass Fiber Filter)
• Elution Volume: 50–120 μL
• Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Product Type: RNA Isolation Kit
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Starting Material Amount: Bacteria: ≤10⁹ cells, Cells: ≤10⁷, Tissue: ≤75 mg, Plant: ≤75 mg, Yeast: ≤10⁸ cells, Virus: varies, Enzymatic Reactions: varies

Sikkerhed og håndtering

Produktidentifikator

• RNAqueous Total RNA Isolation Kit

Signalord

• Danger

Farekategori

• Acute toxicity Category 4
• Specific target organ toxicity Category 2
• Specific target organ toxicity Category 3
• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 1B
• Serious eye damage/eye irritation Category 2
• Germ cell mutagenicity Category 2
• Reproductive toxicity Category 1B
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Faresætning

• H302-Harmful if swallowed.
• H371-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H360-May damage fertility or the unborn child.

Sikkerhedssætning

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplerende oplysninger

• MIXTURE LIST-Contain: Lithium chloride, Formamide, Formaldehyde, Methanol, 2-Mercaptoethanol

For Research Use Only. Not for use in diagnostic procedures.