RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 9 publications

Product Code	Brand	Quantity
18799033	Novus Biologicals NB200-598SS	0.025 mL
18736703	Novus Biologicals NB200-598	0.1 mL

Description

RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Flow (Intracellular), CyTOF-ready.

Specifications

• Antigen: RNA Polymerase II/POLR2A
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Chromatin Research, Epigenetics, microRNA, Transcription Factors and Regulators
• Gene Accession No.: P24928
• Gene ID (Entrez): 5430
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 217 kDa

• Clone: 4H8
• Dilution: Western Blot 1-2 ug/ml, Flow Cytometry 1 ug per million cells, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Yeast, Primate (Negative)
• Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
• Gene Symbols: POLR2A
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.'

For Research Use Only