missing translation for 'onlineSavingsMsg'
Learn More

Macherey-Nagel™ RNA Isolation, RA1 Lysis Buffer

Brand:  Macherey-Nagel™ 740961.500

Code : 86

Additional Details : Weight : 0.50000kg


Quantity 500mL

 View more versions of this product

Product Code. 10335832

  • £150.00 / 500mL


Estimated Shipment
14-12-2017
Add to basket


Description

Description

Cells are lysed by incubation in a solution containing chaotropic ions. This lysis buffer inactivates RNases creating appropriate binding conditions favouring adsorption of RNA to the silica membrane. After lysis, homogenisation and reduction of viscosity are achieved by filtration with NucleoSpin™ filter units. Contaminating DNA bound to the membrane is removed by a DNase I solution directly applied to the membrane during preparation. Optimal conditions for DNase are achieved by washing the membrane with a specific desalting buffer before treatment. Salts, metabolites and macromolecular cellular components are removed by simple washing with two different buffers. Total RNA is finally eluted with RNase-free water. The NucleoSpin™ RNA Plant kit features two alternative lysis buffers: in most cases, use of buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi may lead to solidification of the lysate, resulting in non-processible slurry. In such cases, the alternative buffer, RAP is the buffer of choice. For thorough homogenisation and reduction of lysate viscosity, NucleoSpin™ filter units are also provided. Kit components: NucleoSpin™ RNA columns with 2mL collecting tubes, 2mL collecting tubes, 1.5mL microcentrifuge tubes, NucleoSpin™ filters, buffers, RNase-free DNase I, DNase I reaction buffer, RNase-free water.
  • Silica membrane technology
  • Yield: 3μg to 70μg from 100μg plant material
  • Sample material: 1mg to 100mg tissue
  • Elution volume: 60μL
  • Fragment size: 200b to 20kb
  • Binding capacity: 200μg
  • Preparation time: 30min/6 preps
  • Format: mini spin column
  • DNase I included
  • NucleoSpin filter columns included for homogenisation of lysate and reduction of viscosity
  • Parallel purification of DNA possible by using the NucleoSpin RNA/DNA buffer set NucleoSpin™ RNA Plant is designed for isolation of DNA-free high quality RNA from a wide variety of plant and fungal samples.
Specifications

Chemical Identifiers

Lysis Buffer RA1
For RNA isolation

Specifications

For RNA isolation
Lysis Buffer RA1
500mL
Videos
Safety and Handling

Safety and Handling

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H302-Harmful if swallowed.
  • H412-Harmful to aquatic life with long lasting effects.
  • EUH031-Contact with acids liberates toxic gas.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ A§ if you feel unwell.
  • P330-Rinse mouth.

Documentation

Technical Documents