Macherey Nagel™ RNA Isolation, RA1 Lysis Buffer


Overview
Brand: Macherey Nagel™

Manufacturer Part Number: 740961

50 ML LYSIS BUFFER RA1 50ML

UNSPSC: 12161700

Code: 86

Additional Details:
Additional Details: Weight: 0.12500kg


Quantity 50mL

Product Code. 11912412

Quantity Price
1 £ 35.25 / 50mL
Estimated Shipment
04-01-2017
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Description and Specification

Specification

For Use With For RNA isolation
Buffers Lysis Buffer RA1
Quantity 50mL

Cells are lysed by incubation in a solution containing chaotropic ions. This lysis buffer inactivates RNases creating appropriate binding conditions favouring adsorption of RNA to the silica membrane. After lysis, homogenisation and reduction of viscosity are achieved by filtration with NucleoSpin™ filter units. Contaminating DNA bound to the membrane is removed by a DNase I solution directly applied to the membrane during preparation. Optimal conditions for DNase are achieved by washing the membrane with a specific desalting buffer before treatment. Salts, metabolites and macromolecular cellular components are removed by simple washing with two different buffers. Total RNA is finally eluted with RNase-free water. The NucleoSpin™ RNA Plant kit features two alternative lysis buffers: in most cases, use of buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi may lead to solidification of the lysate, resulting in non-processible slurry. In such cases, the alternative buffer, RAP is the buffer of choice. For thorough homogenisation and reduction of lysate viscosity, NucleoSpin™ filter units are also provided. Kit components: NucleoSpin™ RNA columns with 2mL collecting tubes, 2mL collecting tubes, 1.5mL microcentrifuge tubes, NucleoSpin™ filters, buffers, RNase-free DNase I, DNase I reaction buffer, RNase-free water.
  • Silica membrane technology
  • Yield: 3μg to 70μg from 100μg plant material
  • Sample material: 1mg to 100mg tissue
  • Elution volume: 60μL
  • Fragment size: 200b to 20kb
  • Binding capacity: 200μg
  • Preparation time: 30min/6 preps
  • Format: mini spin column
  • DNase I included
  • NucleoSpin filter columns included for homogenisation of lysate and reduction of viscosity
  • Parallel purification of DNA possible by using the NucleoSpin RNA/DNA buffer set NucleoSpin™ RNA Plant is designed for isolation of DNA-free high quality RNA from a wide variety of plant and fungal samples.

Documentation

Technical documents