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Cytiva rmp Protein A Sepharose™ Fast Flow Affinity Media
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Quantity:
5 mL
25 mL
200 mL
Unit Size:
200mL
25mL
Each
Description
- Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
- Coupling chemistry that minimizes leakage of protein A ligand
- Multi-point attachment of rProtein A by chemically-stable amide bonds
- High capacity and good pressure/flow characteristics provide for high throughput
- Matrix: 4% cross-linked agarose
- Average particle size: 90μm (d50V)
- Ligand: recombinant protein A (E. coli)
- pH Stability working range: 3 to 10
- pH Stability cleaning-in-place: 2 to 11
- Binding capacity/mL chromatography medium: >35mg human IgG/mL
- Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column
For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.
Specifications
Specifications
| Quantity | 25 mL |
| Stability | Stable in aqueous Buffers commonly used in Protein A Chromatography |
| For Use With (Application) | For High-Purity Separation of Monoclonal and Polyclonal Antibodies |
| Storage Requirements | 2°C to 8°C |
| Binding Capacity | >Min. 22 mg Human IgG/mL |
| Product Type | rmp Protein A Sepharose Fast Flow Media |
| Particle Size | 60 to 165 μm |
| Material | Recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix |
| Matrix | 4% Cross-Linked Agarose |
| pH Range | 3 to 10 |
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