Cytiva rmp Protein A Sepharose™ Fast Flow Affinity Media

Designed for high-purity separation of monoclonal and polyclonal antibodies

Brand:  Cytiva 17-5138-03

Product Code. 10358374

Description

• Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
• Coupling chemistry that minimizes leakage of protein A ligand
• Multi-point attachment of rProtein A by chemically-stable amide bonds
• High capacity and good pressure/flow characteristics provide for high throughput

• Matrix: 4% cross-linked agarose
• Average particle size: 90μm (d50V)
• Ligand: recombinant protein A (E. coli)
• pH Stability working range: 3 to 10
• pH Stability cleaning-in-place: 2 to 11
• Binding capacity/mL chromatography medium: >35mg human IgG/mL
• Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column

For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.

Specifications

• Quantity: 200 mL
• Stability: Stable in aqueous Buffers commonly used in Protein A Chromatography
• Binding Capacity: >Min. 22 mg Human IgG/mL
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• pH Range: 3 to 10
• Ligand Type: Recombinant Protein A (E. coli)

• For Use With (Application): For High-Purity Separation of Monoclonal and Polyclonal Antibodies
• Storage Requirements: 2°C to 8°C
• Product Type: rmp Protein A Sepharose Fast Flow Media
• Particle Size: 60 to 165 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol