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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq
Beskrivning
The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.
The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology
Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).
Specifikationer
Specifikationer
| Content And Storage | Each kit contains: |
| Isolation Technology | Magnetic Bead |
| Sample Type | Total RNA |
| Final Product Type | Transcriptome RNA |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Application) | Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction |
| No. of Reactions | 8 Preps |
| Product Line | RiboMinus |
| Quantity | 8 Preps (10 μg⁄prep) |
| Sequencing Type | Transcriptome Sequencing |
| Visa mer |
For Research Use Only. Not for use in diagnostic procedures.
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