Rhodopsin, Rabbit anti-Human,Mouse,Rat,Amphibian,Bovine,Zebrafish, Clone: Rho 1D4, Novus Biologicals™

Rabbit Monoclonal Antibody

Product Code	Brand	Quantity
18389410	Novus Biologicals NBP2-81059-0.025MG	0.025 mg
18248128	Novus Biologicals NBP2-81059-0.2MG	0.2 mg

Description

Rhodopsin Monoclonal specifically detects Rhodopsin in Human, Mouse, Rat, Amphibian, Bovine, Zebrafish samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay.

Specifications

• Antigen: Rhodopsin
• Concentration: 1 mg/mL
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmune Assays (RIA)
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: GPCR, Phospho Specific, Signal Transduction, Vision
• Formulation: PBS
• Gene ID (Entrez): 6010
• Immunogen: Bleached bovine rod outer segment (ROS).
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Clone: Rho 1D4
• Dilution: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
• Gene Alias: CSNBAD1, MGC138309, OPN2MGC138311, opsin 2, rod pigment, opsin-2, retinitis pigmentosa 4, autosomal dominant, rhodopsin, RP4
• Gene Symbols: RHO
• Isotype: IgG κ
• Purification Method: Protein A purified
• Test Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.