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Rhodopsin Antibody (Rho 1D4), Alexa Fluor™ 700, Novus Biologicals™

Product Code. 18348780 Shop All Bio Techne Products
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Product Code. 18348780 Supplier Novus Biologicals Supplier No. NBP281059AF700

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Description

Rabbit Monoclonal Antibody

Rhodopsin Monoclonal specifically detects Rhodopsin in Human, Mouse, Rat, Amphibian, Bovine, Zebrafish samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Rhodopsin
Applications Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
Classification Monoclonal
Clone Rho 1D4
Conjugate Alexa Fluor 700
Dilution Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence
Formulation 50mM Sodium Borate
Gene Alias CSNBAD1, MGC138309, OPN2MGC138311, opsin 2, rod pigment, opsin-2, retinitis pigmentosa 4, autosomal dominant, rhodopsin, RP4
Gene Symbols RHO
Host Species Rabbit
Immunogen Bleached bovine rod outer segment (ROS).
Purification Method Protein A purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline GPCR, Phospho Specific, Signal Transduction, Vision
Primary or Secondary Primary
Gene ID (Entrez) 6010
Test Specificity The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Target Species Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
Content And Storage Store at 4°C in the dark.
Form Purified
Isotype IgG κ
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