Invitrogen™ RGS14 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA597309

Product Code. 16550142

Description

Immunogen sequence: PPVEGPGGRP LRKSFRRELG GTANAALRRE SQGSLNSSAS LDLGFLAFVS SKSESHRKSL GSTEGESESR PGKYCCVYLP DGTASLALAR PGLTIRDMLA GICEKRGLSL PDIKVYLVGN EQALVLDQDC TVLADQEVRL ENRITFELEL TALERVVRIS AKPTKRLQEA LQPILEKHGL SPLEVVLHRP GEKQPLDLGK LVSSVAAQRL VLDTLPGVKI SKARDKSPCR SQGCPPRTQD KATHPPPASP SSLVKVPSSA TGKRQTCDIE GLVELLNRVQ S; Positive Samples: SH-SY5Y, Mouse kidney; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, PML body, centrosome, cytoskeleton, dendrite, dendritic spine, microtubule organizing center, postsynaptic cell membrane, postsynaptic density, spindle, spindle pole, synapse.

RGS14 is a member of the regulator of G-protein signaling family which contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. RGS14 attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. The resulting hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of RGS14 have been observed but have not been thoroughly characterized. Further, RGS14 acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism; plays a role in cell division; is required for the nerve growth factor (NGF)-mediated neurite outgrowth, and is involved in stress resistance. RGS14 protein may also be involved in visual memory processing capacity and hippocampal-based learning and memory.

Specifications

• Antigen: RGS14
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rgs14
• Gene Alias: rap1/rap2 interacting protein; RAP1/RAP2-interacting protein; Regulator of G-protein signaling 14; regulator of G-protein signalling 14; RGS14; RGSE; RPIP1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10636, 114705, 51791
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.4 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O08773, O43566, P97492
• Gene Symbols: Rgs14
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-510 of human RGS14 (NP_0064712).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG