Invitrogen™ RFWD2 Recombinant Rabbit Monoclonal Antibody (2R8A5)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA557923

Product Code. 30284002

Description

Immunogen sequence: YYDLRNTKQP IMVFKGHRKA VSYAKFVSGE EIVSASTDSQ LKLWNVGKPY CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV.

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). [UniProt].

Specifications

• Antigen: RFWD2
• Classification: Recombinant Monoclonal
• Concentration: 1.01 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene Accession No.: Q8NHY2, Q9R1A8
• Gene Symbols: RFWD2
• Immunogen: Recombinant protein (or fragment).
• Quantity: 20 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 2R8A5
• Conjugate: Unconjugated
• Gene: RFWD2
• Gene Alias: AI316802; C80879; Constitutive photomorphogenesis protein 1 homolog; constitutive photomorphogenic protein 1; COP1; COP1D; E3 ubiquitin-protein ligase COP1; E3 ubiquitin-protein ligase RFWD2; hCOP1; mCOP1; putative ubiquitin ligase COP1; Rfwd2; RGD1304773; ring finger and WD repeat domain 2; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2; RNF200
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 26374, 360860, 64326
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid