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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL)

A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

Brand:  Thermo Scientific™ EP0451

126.75 GBP valid until 2022-08-31
Use promo code "16497" to get your promotional price.



Code : 88

Additional Details : Weight : 0.97980kg

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Product Code. 10121360

  • £143.00 / 10000 units
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Description

Description

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45°C
  • Active up to 55°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications

Specifications

RevertAid H Minus Reverse Transcriptase (200 U/μL) is supplied with 5X Reaction Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT).
Store at -20°C.
1mL 5X Reaction Buffer
RevertAid
RevertAid H Minus
13 kb or Less
42°C to 45°C
10,000 units
None
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