Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1

Product Code. 12756553

Brand: Macherey-Nagel™ 740911.1

Description

For use in NucleoSpin™ Plasmid DNA purification kits for rapid small-scale preparation of high-copy plasmids from E. coli. Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1 without RNase A is a replacement component used in the NucleoSpin™ method's cell lysis process to resuspend the cell pellet.

The NucleoSpin™ Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mL E. coli overnight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.

After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing, highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used.

Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, all necessary buffers, RNase A, MN wash plate, MN tube strips, cap strips.

• Silica-membrane technology
• Yield: up to 20μL sequencing grade plasmid DNA
• Elution volume: 75μL to 150μL
• Culture volume: 1mL to 5mL
• Binding capacity: 25μg
• Maximum plasmid size <15kb
• No use of organic solvents
• NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included

For further information on this product contact your local Customer Services.

Specifications

• Chemical Name or Material: Resuspension Buffer
• Color: Colorless
• For Use With (Application): For Plasmid DNA Purification
• For Use With (Equipment): NucleoSpin™ Plasmid DNA Purification Kits
• pH: 7.5to 8.5
• Physical Form: Solution
• Quantity: 1000 mL
• Odor: Odorless