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Invitrogen™ Renin Human ELISA Kit

Sandwich ELISA Kit

£451.00 - £3070.00

Specifications

Accession Number P00797
Assay Range 93.8-6000pg/mL
Assay Sensitivity 32.6 pg/ml
Conjugate Biotin
Product Type ELISA Kit
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Product Code Brand Quantity Price Quantity & Availability  
16312014
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Invitrogen™
BMS2271
96 Tests
£451.00
Pack of 96
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16322014
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Invitrogen™
BMS2271TEN
96 x 10 Tests
£3070.00
Pack of 96
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Description

Description

The Renin Human ELISA Kit quantitates Hu Renin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Renin.

Principle of the method

The Human Renin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Renin is an enzyme secreted by the kidney that is part of a physiological system that regulates blood pressure. In the blood, renin acts on a protein known as angiotensinogen, resulting in the release of angiotensin I. Angiotensin I is cleaved by angiotensin-converting enzyme, splitting off two amino acids from the 10-amino-acid chain of angiotensin I, to form angiotensin II. The resultant angiotensin II octapeptide acts via receptors to constrict arterioles, causing a rise in both systolic and diastolic blood pressure. Angiotensin II is one of the most active vasoconstrictors known; on a weight basis it is significantly more potent than norepinephrine. It also increases the secretion of cortisol and aldosterone by a direct action on the adrenal cortex.
Specifications

Specifications

P00797
32.6 pg/ml
ELISA Kit
Human
5972
5.1%
Pre-coated 96-well Plate, Standard (2 vials), Sample Diluent, Assay Buffer Concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
1 hr. 20 min.
93.8-6000pg/mL
Biotin
Cell Lysates, Plasma, Serum
Colorimetric Microplate Reader
REN
3.9%
HRP
Serum, 50 μL; Plasma, 50 μL; Cell Lysate,50 μL
Human
3 hr. 30 min.
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