Invitrogen™ Renin 1 (REN1) Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMREN1X5

Product Code. 17165769

Description

The Mouse Renin 1 (REN1) ELISA quantitates Ms Renin 1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Renin 1. Principle of the method The Mouse Renin 1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Specifications

• Accession Number: P06281
• Assay Sensitivity: 6 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 19701
• Interassay CV: <12%
• Kit Contents: Standard, Adhesive Plate Covers, Wash Buffer, Assay Diluent Concentrate, Chromogen, SAV-HRP, Stop Solution, Biotinylated Detection Antibody, Pre-coated 96 well plates
• Quantity: 5 x 96 Tests
• Research Discipline: Neurobiology
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 12.21 pg/mL to 50,000 pg/mL
• Calibration Range: 6 pg/mL to 50000 pg/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA antibody pair detects mouse Renin. Other species not determined yet
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: D19352,Ren,Ren-1,Ren-Ac,Ren1d,Rn-1,Rnr,Ren1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L
• Synonym: D19352, Ren, Ren-1, Ren-Ac, Ren1d, Rn-1, Rnr, Ren1
• Test Time: 1 hr. 20 min.