Invitrogen™ Renilla luciferase Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA532210

Product Code. 13496727

Description

Recommended positive controls: 293T transfected Renilla Luciferase. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Renilla luciferase (RLuc), derived from the sea pansy Renilla reniformis, is a widely-used reporter gene in molecular biology for in vitro and in vivo assays, including gene expression studies and small animal imaging. The luciferase enzyme, approximately 37 kDa in size, belongs to the alpha/beta hydrolase family and operates via a catalytic mechanism involving the oxidative decarboxylation of its substrate, coelenterazine, in the presence of molecular oxygen, resulting in the emission of blue light. The gene encoding Renilla luciferase has been isolated and characterized, with its cDNA available for various applications. Structurally, RLuc contains a hydrolase fold akin to that of bacterial dehalogenases, suggesting an evolutionary relationship. Despite extensive utilization, the exact details of its active site and catalytic mechanism continue to be explored. The bioluminescent property of RLuc is particularly useful for generating a detectable signal in various biological assays, enhancing its utility as a biosensor.

Specifications

• Antigen: Renilla luciferase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: Renilla-luciferin 2-monooxygenase
• Immunogen: Full length Renilla Luciferase recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Free Floating), Western Blot, Immunocytochemistry
• Concentration: 1.14 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Luciferase
• Product Type: Antibody
• Isotype: IgG