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Thermo Scientific™ Oxichrome Reagent
Description
Rapidly detect the enzyme cytochrome oxidase in microorganisms using Oxichrome Reagent.
Rapidly detect the enzyme cytochrome oxidase in microorganisms using Thermo Scientific™ Remel™ Oxichrome Reagent. The oxidase test was originally developed to identify Neisseria spp., but later was used to separate pseudomonads from oxidase-negative enteric Gram-negative bacilli (Enterobacteriaceae)1,2. Most Gram-positive bacteria are oxidase-negative, pseudomonads are oxidase-positive, and other non-enteric, Gram-negative bacilli, are variable
Pseudomonads, bacteria of the family Pseudomonadaceae are a large and varied group comprising four major genera and several hundred species. Most species of the family are found in soil or water; some cause diseases in plants, and a few cause serious diseases in humans and other mammals. Pseudomonas aeruginosa, very common and widespread, is an opportunistic pathogen for man that causes antibiotic-resistive infections in persons of weakened resistance.
The oxidase reaction is attributed to the presence of a cytochrome oxidase system in the respiratory chain of certain bacteria, which utilizes molecular oxygen as a terminal electron acceptor3,4. Oxidase-positive bacteria produce oxidase enzymes which, in the presence of atmospheric oxygen, cytochrome c, and an oxidase reagent (Oxichrome Reagent), oxidize the reagent to form a blue-purple colored compound, Wurster’s blue.
- Ready-to-use – Liquid reagent
- Easy to interpret – Blue to purple color indicates a positive test
- Easy-to-store – Storage at room temperature
- Rapid results – Can be interpreted within 30 seconds
Oxichrome reagent contains N,N,N,N-tetramethyl-ρ-phenylenediamine supplied in 15 mL bottles.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
Specifications
| Content And Storage | Store at Room Temperature Until Used |
| Description | Oxichrome Reagent |
| Format | Vial |
| Packaging Type | 15 mL/Vial |
| Quantity | 15 mL/Vial |
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