Constitutive expression for controls in Red Firefly luciferase assays
The Red Firefly Luc Vectors contain a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.
Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin
Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization