R&D Systems™ Recombinant S. plicatus Endo H Protein

Product Code.
18349257	50ug; Carrier Free

Product Code. 18349257 Supplier R&D Systems™ Supplier No. 5224GHB050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to deglycosylate ribonuclease B under native conditions. The DC50 is <5 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 μg of RNase B in 30 minutes at 37 °C. . Use of Recombinant S. plicatus Endo-beta-N-acetylglucosaminidase H/Endo H in the deglycoslyation of other substrates may require alternative conditions for optimal performance.

Specifications

• Accession Number: P04067
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
• Molecular Weight (g/mol): M.W. (Observed): 29 kDa, reducing conditions; M.W. (theoretical): 30 kDa
• Name: Endo-beta-N-acetylglucosaminidase H/Endo H
• Quantity: 50 μg
• Source: E. coli-derived s. plicatus Endo-beta-N-acetylglucosaminidase H/Endo H protein Val47-Pro313 with an N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Endo-beta-N-acetylglucosaminidase H/Endo H