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R&D Systems™ Recombinant S. cerevisiae SUMO Protease ULP1 His Protein, CF
Description
Measured by its ability to cleave SUMO-GFP Protease Substrate. The DC50 is <0.8 ng, as measured under the described conditions.The DC50 is defined as the amount of enzyme required to cleave 50% of 1 μg SUMO-GFP in 2 hours at room temperature. Use of Recombinant Yeast SUMO Protease ULP1 in the cleavage of other protein substrates may require alternative conditions for optimal performance.
Specifications
Specifications
| Accession Number | NP_015305.1 |
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol. |
| Molecular Weight (g/mol) | M.W.-observed: 27-33 kDa, under reducing conditions., M.W.-theroretical: 26 kDa |
| Quantity | 50 μg |
| Source | E. coli-derived yeast SUMO Protease ULP1 protein Leu403-Lys621, with an N-terminal Met and 6-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Conjugate | Unconjugated |
| Protein | SUMO-GFP Protease Substrate |
Product Title
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