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R&D Systems™ Recombinant Mouse Tryptase beta-1/Mcpt7 Protein
Description
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >75 pmol/min/μg, as measured under the described conditions.
Specifications
Specifications
| Accession Number | Q02844 |
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Gene ID (Entrez) | 17230 |
| Molecular Weight (g/mol) | M.W. (Observed): 36 kDa and 37 kDa, reducing conditions; M.W. (theoretical): 28.8 kDa |
| Name | Tryptase beta-1/MCP-7/Mcpt7 |
| Quantity | 20 μg |
| Source | Mouse myeloma cell line, NS0-derived mouse Tryptase beta-1/MCP-7 protein Ile29-Phe273, with an C-terminal 10-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Structural Form | Mature form |
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