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R&D Systems™ Recombinant Mouse Syndecan-1 Protein
Description
Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. When 5 x 104 cells/well are added to mouse Syndecan-1 and Recombinant Human Fibronectin (0.5 μg/mL, Catalog # 4305-FNB ) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 2.5-10 μg/mL.
Specifications
Specifications
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS. |
| Gene ID (Entrez) | 20969 |
| Molecular Weight (g/mol) | 25.3 kDa |
| Name | Syndecan-1/CD138 |
| Quantity | 50 μg |
| Source | Mouse myeloma cell line,NS0-derived mouse Syndecan-1/CD138 protein Gln18-Glu252,with a C-terminal 6-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. |
| Conjugate | Unconjugated |
| Recombinant | Recombinant |
| Purity or Quality Grade | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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