R&D Systems™ Recombinant Mouse Sonic Hedgehog/Shh GMP Protein, CF

Product Code. 30564810

Brand: R&D Systems™ BTSHHGMP500

Description

Recombinant Protein

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.0500-0.600 μg/mL.

Specifications

• Accession Number: Q62226.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate, NaCl and DTT with Trehalose.
• Gene ID (Entrez): 6469
• Molecular Weight (g/mol): Observed: 21 kDa, under reducing conditions.,Theoretical: 19.8 kDa
• Quantity: 500 μg
• Source: E. coli-derived mouse Sonic Hedgehog/Shh protein Cys25 - Gly198 (Cys25Ile-Ile) with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidlines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile water.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 19844 Da ± 26 Da
• Protein: Sonic Hedgehog/Shh