R&D Systems™ Recombinant Mouse Pref-1/DLK-1/FA1 Protein

Product Code. 18717597

Brand: R&D Systems™ 8545PR050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse Pref-1/DLK-1/FA1 Protein is derived from NS0. The Recombinant Mouse Pref-1/DLK-1/FA1 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q09163
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 8788
• Molecular Weight (g/mol): Observed M.W.: 49-66kDa, reducing conditions, Theoretical M.W.: 31kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse Pref-1/DLK1/FA1 protein Ala24-Gln305,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: delta-like 1 homolog (Drosophila), DLK, DLK1, DLK-1, FA1, FA1fetal antigen 1, pG2, pG2delta-like homolog (Drosophila), preadipocyte factor 1, Pref-1, PREF1, protein delta homolog 1, secredeltin, ZOG
• Common Name: Pref-1/DLK1/FA1
• Gene Symbol: Dlk1
• Biological Activity: Measured by its ability to inhibit adipocyte differentiation of mouse 3T3-L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725. Recombinant Mouse Pref-1/DLK1/FA1 at 2.5μg/mL will inhibit adipocyte differentiation by more than 50%.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Ala24-Gln305, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining