R&D Systems™ Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein

Product Code. 18378957

Brand: R&D Systems™ 796IC050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM/Fc Chimera coated plates (12.5 μg/mL with 100 μL/well), approximately 65-95% will adhere after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: Q3U8M7
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3383
• Molecular Weight (g/mol): M.W. (Observed): 110-130 kDa, reducing conditions; M.W. (theoretical): 76.8 kDa (monomer)
• Name: ICAM-1/CD54 Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse ICAM-1/CD54 protein Mouse ICAM-1 (Gln28-Asn485) (N-terminus) Accession # Q3U8M7 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Icam1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 0.4 mg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ICAM-1/CD54