R&D Systems™ Recombinant Mouse Granzyme B Protein

Product Code.	Quantity	unitSize
18344947	10 μg	10µg

Product Code. 18344947 Supplier R&D Systems™ Supplier No. 1865SE010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >2,000 pmol/min/μg as measured under the described conditions.

Specifications

• Accession Number: P04187
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2.
• Gene ID (Entrez): 3002
• Molecular Weight (g/mol): M.W. (Observed): Multiple bands between 34-38 kDa, reducing conditions; M.W. (theoretical): 27 kDa
• Name: Granzyme B
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Granzyme B protein Lys17-Ser247, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Structural Form: Pro form
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Gzmb
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Granzyme B