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R&D Systems™ Recombinant Mouse Endostatin Protein
Description
Measured by its ability to inhibit the FGF basic/FGF2-dependent proliferation of HUVEC human umbilical vein endothelial cells. Dhanabal, M. et al. (1999) Biochem. Biophys. Res. Commun. 258:345. The ED50 for this effect is 0.3-1.5 μg/mL in the presence of 20 μg/mL of Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570 ).
Specifications
Specifications
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS. |
| Gene ID (Entrez) | 12822 |
| Molecular Weight (g/mol) | 21.3 kDa |
| Name | Endostatin |
| Quantity | 50 μg |
| Source | Mouse myeloma cell line,NS0-derived mouse Endostatin protein His1591-Lys1774,with an N-terminal Met,a substitution,Leu1684Val and a C-terminal 6-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. |
| Conjugate | Unconjugated |
| Recombinant | Recombinant |
| Purity or Quality Grade | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
Product Title
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