R&D Systems™ Recombinant Mouse CD8 alpha/beta Heterodimer Protein, CF

Product Code. 18775366

Brand: R&D Systems™ 11146CD050

Description

Analyzed by SEC-MALS.

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.750-6.00 μg/mL.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 925
• Molecular Weight (g/mol): MolecularWeight-observed: 33-37 kDa & 45-51 kDa, under reducing conditions., MolecularWeight-theroretical: 28 kDa (CD8 alpha) & 27 kDa (CD8 beta)
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived mouse CD8 protein Mouse CD8 alpha (Lys28-Tyr196) (N-terminus) Accession # NP_033987.1 Acidic Tail 6-His tag Mouse CD8 beta (Leu22-Thr175) (N-terminus) Accession # P10300.1 Basic Tail FLAG (DYKDDDDK) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CD_antigen: CD8a, CD8, CD8 antigen, alpha polypeptide (p32), CD8A, CD8a molecule, LEU2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD8