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R&D Systems™ Recombinant Mouse BID Protein
Description
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria. The typical EC50 for cytochrome c releasing activity is less than 300 nM. Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine™ ELISA Kit (Catalog # MCTC0 ). The EC50 for the desired application should be determined. Recombinant Mouse BID Caspase-8-cleaved is available (Catalog # 883-M8 ).
Specifications
Specifications
| Accession Number | AAC71064 |
| For Use With (Application) | Bioactivity |
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES and KCl. |
| Gene ID (Entrez) | 637 |
| Molecular Weight (g/mol) | M.W. (Observed): 22 kDa, under reducing and non-reducing conditions; M.W. (theoretical): 22 kDa |
| Name | BID |
| Quantity | 50 μg |
| Source | E. coli-derived mouse BID protein Met1-Asp195 |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Structural Form | Monomer and disulfide-linked homodimer |
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