R&D Systems™ Recombinant MERS-CoV 3CL Protease

Product Code. 18756106

Brand: R&D Systems™ E719050

Description

Carrier Free Recombinant Protein

Recombinant MERS-CoV virus 3CL Protease is a cysteine protease that cleaves proteins with sequences including a LQ[S/A/G]. The cleavage occurs c-terminal to the glutamine residue. We recommend an initial MERS-CoV 3CL Protease concentration of 50-100 nM when using substrate S-720.

Specifications

• Accession Number: K9N638
• For Use With (Application): Bioactivity
• Formulation: 1.7 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT
• Gene ID (Entrez): 43740578
• Molecular Weight (g/mol): M.W.-theoretical: 33 kDa
• Quantity: 50 μg
• Source: E. coli-derived mers-cov 3 CL Protease protein Ser3248 - Gln3553 Contains the following substitutions: Val 3551 to Leu; Met 3552 to Phe
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 48 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: 3 CL Proteinase, 3 C-like main protease, 3 CL-Mpro, COVID-19, M Proteinase, Main Protease, Mpro
• Conjugate: Unconjugated
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: SARS-CoV-2 3 CL Protease