R&D Systems™ Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

Product Code. 18718668

Brand: R&D Systems™ E305025

Description

Recombinant Protein

Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.

Specifications

• Accession Number: P22314.3
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a solution in HEPES, NaCl and TCEP.
• Gene ID (Entrez): 7317
• Molecular Weight (g/mol): MolecularWeight-theroretical: 118 kDa
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1
• Gene Symbol: UBA1
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: Ubiquitin-activating Enzyme/UBE1