Novus Biologicals™ Recombinant Human Sonic Hedgehog/Shh Protein

Product Code. 18677215

Brand: Novus Biologicals™ NBP235265500ug

Description

Recombinant Protein

Sonic Hedgehog/Shh protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 x 10^3 IU/mg

Specifications

• Accession Number: Q15465, Q15465, Q15465
• For Use With (Application): SDS-PAGE, Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Gene ID (Entrez): 6469
• Molecular Weight (g/mol): MolecularWeight-theroretical: 19.8 kDa
• Quantity: 500 μg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.)
• Endotoxin Concentration: Less than 1 EU/μg of Sonic Hedgehog/Shh as determined by LAL method.
• Gene Alias: HHG1, HHG-1, HLP3, HPE3, MCOPCB5sonic hedgehog (Drosophila) homolog, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
• Gene Symbol: SHH
• Reconstitution: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.)
• Research Category: Biologically Active Proteins
• Purity or Quality Grade: >98 % pure by SDS-PAGE and HPLC
• Protein: Sonic Hedgehog/Shh